고농도 EGF로 처리된 KUMA3 세포주의 세포유전학적 연구

Other Titles
Cytogenetic Studies of High Dose EGF Treated KUMA3 Cell Clone
Authors
채한수송달원정현수김희준홍창의안병훈최인장
Department
Dept. of Otorhinolaryngology (이비인후과학); Dept. of Anatomy (해부학)
Issue Date
1998
Citation
대한이비인후과학회지, Vol.41(2) : 232-237, 1998
ISSN
1225-035X
Abstract
Background and Objectives : Epidermal growth factor (EGF), directly stimulates epidermal growth and differentiation. The combination of EGFR activation and genetic alternations may lead to neoplasia and metaplasia. To study the change of chromosomal number and the aberrations of chromosomal structure of KUMA3 cell line treated with high dose EGF. Materials and Methods : The high dose EGF treated cell clones were obtained from KUMA3 cell line which was established from squamous cell carcinoma of the lower lip by culturing cells in medium containing high dose EGF for 6 months. The chromosomal analysis and subculture were performed at subsequent passage of 1 month interval. Results : In high dose EGF treated cell clones, there was no apparent change in chromosomal number, but the ratio of the number of chromosome 7 to mode chromosome number was similar to normal value (0.043). The new chromosomal structural aberrations appeared first from 30 passage of IR-200 cell clone. The chromosomal aberrations were del (1)(q23 → qter) and del (4)t (1 : 4) (1qter → 1q23 : : 4p16 → 4qter). Conclusion : There was no change in chromosomal number, but the ratio of the number of chromosome 7 to mode chromosome number was similar to normal value (0.043), and the new chromosomal structural aberrations were appeared.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/38333
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
Keimyung Author(s)
송달원; 안병훈; 최인장
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