폐렴연쇄구균을 이용한 생쥐에서의 만성 비부비동염 실험모델

Other Titles
A Mouse Model of Chronic Rhinosinusitis Induced by Streptococcus Pneumoniae
Authors
안병훈박연호김동은송인혁남영진남성일김상표류남희
Department
Dept. of Otorhinolaryngology (이비인후과학); Dept. of Pathology (병리학); Dept. of Laboratory Medicine (진단검사의학)
Issue Date
2006
Citation
대한이비인후과학회지, Vol.49(1) : 52-59, 2006
ISSN
1225-035X
Abstract
Background and Objectives:A mouse has a great potential to be used in studying genetics and inflammatory process of the rhinosinusitis. The aim of this study was to observe effects of experimentally induced chronic rhinosinusitis on histopathology of the sinonasal mucosa in a mouse and to develop a chronic form of rhinosinusitis. Materials and Method:Thirty five, six-week old male C57BL/6 mice were used as follows:7 normal controls without intervention, 7 Sham operated controls, 7 animals with ostial obstruction alone using Merocel, 7 animals implanted with Merocel plus 106 colony forming unit (CFU)/mL of Streptococcus pneumoniae and 7 animals implanted with Merocel plus 108 CFU/mL of S. pneumoniae. Six weeks after intervention, the animals were sacrificed and serially sectioned at 1 mm intervals and stained with Hematoxylin and Eosin. Results:Increased epithelial thickness, goblet cell hyperplasia, epithelial disarray and inflammatory infiltration were observed in the experimental sinuses packed with Merocel alone or Merocel with bacterial inoculation, especially at the nasal septal area. However, there were no significant differences between the Merocel only inserted group and Merocel and bacteria inoculated group. Conclusion:Maxillary sinus ostial obstruction or ostial obstruction with S. pneumoniae inoculation induced chronic rhinosinusitis in C57BL/6 mice as indicated by the histologic change. This study could be used as a model of chronic rhinosinusitis for further study.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/38395
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Otorhinolaryngology (이비인후과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
Keimyung Author(s)
안병훈; 김동은; 남성일; 김상표; 류남희
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