양막 및 제대에서 Endothelin의 조직면역화학

Abstract

Endothelin was originally identified as a product of endothelial cells, which had a paracrine action on vascular smooth muscle, but it is now also known to be product of certain types of epithelial cells. Amnion (n=10) and umbilical cord (n=10) at preterm, term, preeclampsia were subjected to immunochemistry with antiserum cross activity with endothelin-1. Our objective was determine the localiztion immunoreactive endothelin in umbilical cord and amnion during preterm, term, preeclampsia. Intensity of positive staining was given a score of 0(negative),1(low), 2(moderate), or 3(strongly positive) for each group within each section. Comparision of immunochemistry with endothelin from different group(preterm, term preeclampsia) were conducted with Kruskal Wallis one way analysis. Comparision of each section at preterm, term, preeclampsia were conducted with Bonferroni adjusted Mann Whitney test. The intense immunochemistry was present on amnionic epithelial cells, endothelium of umbilical vein, cord epithelium at each group. No difference were detected between each group.