반정량 중합효소연쇄반응에 의한 인형거대세포바이러스 검출

Abstract

Background :Human cytomegalovirus (HCMV) is one of the major etiologic agents of severe illness arid death in immunocompromised patients. Some techniques, such as serology, virus culture, HCMV antigen test and DNA hybridization methods have been developed to detect HCMV infection but there are seyeral drawbacks in them. The polymerase chain reaction (PCR) technique for detecting HCMV DNA, although it promises a high sensitivity, risks the possibility of detecting latent HCMV infection and leading to false-positive results. Thus, we performed semiquantitation of PCR-amplified DNA, so viral load could be assessed by measuring the DNA titer. Method :We performed amplification of HCMV DNA using nested PCR and shell vias assay of peripheral blood, urine and BAL from forty-eight transplant recipients or immunocompromised patients. And semiquantitative PCR assay is performed in samples positive for HCMV DNA. Result :Eighteen (37.5%) of the 48 patients were positive for HCMV DNA in PCR assay, twelve (25.0%) for HCMV in shell trial assay, two(6.4%) of the 31 patients for HCMV IgM antibodies and 26 (83.9%) for HCMV IgG antibodies. In semiquantitative PCR, DNA titer in specimens positive for HCMV in shell vial assay were much higher than negative. Conclusion :We think PCR has the potential to be an effective and reliable procedure for a rapid diagnosis of HCMV infection and a measure of viral load might make it possible to establish the degree of viral burden associated wish the development of clinical symptoms.