한국인 양수의 정균 및 살균작용

Abstract

Forty-four samples of amniotic fluid were studied to evaluate antibacterial activity, of which fifteen samples in midtrimester pregnancy and twenty-nine samples in term were examined by means of colony count method. The antibacterial act1vity of amniotic fluid was analyzed using three pathogenic organisms Staphylococcus aureus(S. aureus, ATCC 25923), Escherchia coli(E. coli, ATCC 25922) and Pseudomonas aeruginosa(P. aerusinosa, ATCC 27853). It was found that antibacterial activity of amnoitic fluid in term pregnancy was higher than in midtrimester. Out of 15 cases in midtrimester, 3(20.0 %) had only bacteriostatic activity against S. aureus, where as the rest had no antibacterial activity against E. coli and P. aeruginosa. 2 out of 29 cases (6.9 %) in term pregnancy had bactericidal activity against S. aureus. Bacterio­static activity against S. auresu, E. coli and P. aeruginosa were 51.7 %, 34.5 % and 9 .1 %, respectively with a view to type of strains, bacteriostatic samples against P. aeruginosa had same activity against E. coli and S. aureus, and stimulatory samples against S. aureus had same activity against E. coli and P. aeruginosa. In comparison with amniotic fluid, tryptic soy borth (TSB) had greater stimulatory effect and distilled water had same bacteriostatic activity against each strain.