계명대학교 의학도서관 Repository

배양된 피부 각질형성세포에서 nuclear factor kB(NFkB)와 AP-1의 활성도에 대한 자외선 B의 효과

Metadata Downloads
Affiliated Author(s)
류영욱이규석
Alternative Author(s)
Ryoo, Young WookLee, Kyu Suk
Journal Title
대한피부과학회지
ISSN
0494-4739
Issued Date
1997
Keyword
UVB irradiationKeratinocytesFibroblastsAP-1NF-kB
Abstract
Background: Ultraviolet B(UVB) light, which can cause severe damage like induction and promotion of cancer, cutaneous inflammation and immunosuppression, represents one of the most important environmental impacts for humans. Keratinocytes are natural target cells of UVB in humans. NF-kB plays a role in the cell of the immune system, where it controls the expression of various cytokines and the major histocompatibility complex gene. Objective : The purpose of this study was to examine the effect of UVB on the NF-kB and AP-1 activity in cultured human keratinocytes and fibroblasts. Methods : Keratinocyte and fibroblast cultures were produced with DMEM medium. Cells were irradiated for 100J/m, 200J/m, 300 J/m. and Nuclear proteins were extracted. AP-1 and NF-kB activities were measured by the gel shift mobility assay. Results : Gel shift mobility assay. 1. The NFkB activity was increased upon UVB in a dose dependent manner in the keratinocyte. 2. Enhanced levels of AP-1 binding activity in the radiated extracts frorn human skin keratinocytes were detected. 3. The levels of GRE(glucocorticoid responsive element) binding activity were similar in both radiated and unradiated extracts from fibroblasts and keratinocytes. Conclusions : The activities of NF-aB and AP-1 are increased following stimulation of a cell with UVB irradiation. Therefore, UV induced skin tumors, abnormal cell proliferation, cutaneous inflammation and immunosuppression, may be due to these transcriptional factors.
Alternative Title
The Effect of UVB on the NF-kB and AP-1 Activity in Cultured Human Keratinocytes and Fibroblasts
Department
Dept. of Dermatology (피부과학)
Publisher
School of Medicine
Citation
구본식 et al. (1997). 배양된 피부 각질형성세포에서 nuclear factor kB(NFkB)와 AP-1의 활성도에 대한 자외선 B의 효과. 대한피부과학회지, 35(3), 411–417.
Type
Article
ISSN
0494-4739
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/39823
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.