계명대학교 의학도서관 Repository

자외선 B 조사에 따른 HaCaT 각질형성세포주 및 편평상피세포암 세포주에서 Anti-proliferative Genes의 발현 양상 비교

Metadata Downloads
Author(s)
김창욱조재위이규석
Alternative Author(s)
Kim, Chang WookCho, Jae WeLee, Kyu Suk
Publication Year
2006
Keyword
Anti-proliferative genesDNA damageHaCaT cellsUVB
Abstract
Background: Impairment of the DNA repair system after UVB irradiation is the key factor in transformation of
normal keratinocytes to squamous cancer cells (SCC). Among anti-proliferative genes (APRO), BTG1, BTG2, and
TOB genes are involved in the repairing of DNA damage and, BTG3 and TOB 2 are involved in differentiation and
cell cycle arrest in human cells, respectively. However, the expressions of APRO genes in SCC and human keratinocytes
after UVB irradiation are largely unknown.
Objective: The purpose of this study was to investigate the expression levels of APRO genes in UVB-irradiated
HaCaT keratinocyte lines and SCC cell lines.
Methods: Cells were irradiated with UVB at various doses (0, 100, 200, 300 mJ/cm2), then total RNA was extracted
from the cells. The expression of genes were confirmed by RT-PCR analysis using specific primers. The
results were obtained from two independent experiments.
Results: The expression of BTG1, BTG2, and TOB2, but not BTG3 and TOB, were markedly increased in
UVB-irradiated HaCaT cells, compared to UVB-irradiated SCC. To confirm the expression levels of BTG1, BTG2,
and TOB2 genes correlated with extent of DNA damage or susceptibility to cell death by UVB, we carried out
staining of surviving UVB-irradiated HaCaT and SCC cell lines. Interestingly, it was found that the number of
surviving HaCaT colonies was higher than SCC cell lines.
Conclusion: The number of surviving colonies, as well as the expression of BTG1, BTG2, and TOB2 after UVB
irradiation were higher in HaCaT cells than the SCC cell lines, suggesting that the gene expression of BTG1,
BTG2, and TOB2 in HaCaT cells might be involved in reducing the death of UVB-irradiated cells by repairing
DNA damage.
Alternative Title
Comparison of the Expression Levels of Anti-proliferative Genes between HaCaT
Keratinocytes and Squamous Cancer Cell Lines after UVB Irradiation
Department
Dept. of Dermatology (피부과학)
Publisher
School of Medicine
Citation
대한피부과학회지, Vol.44(11) : 1317-1324, 2006
Type
Article
ISSN
0494-4739
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/39861
Authorize & License
  • AuthorizeOpen
  • EmbargoForever
Files in This Item:

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.