프락셔널 레이저(Er:glass) 조사한 무모생쥐에서 교원질의 합성 및 TGF-β1의 발현
- Other Titles
- Collagen Synthesis and Expression of TGF-β1 in Er:glass Fractional Laser Treated Hairless Mice
- 김성애; 이대식; 김광훈; 김세르게이; 이규석
- Dept. of Dermatology (피부과학)
- Issue Date
- 대한피부과학회지, Vol.48(2) : 79-86, 2010
- Background: Non-ablative dermal remodeling and ablative skin resurfacing are currently well-established skin
treatment modalities. Fractional laser was recently introduced as a new concept for laser skin rejuvenation, and is
characterized by creation of a dense pattern of epidermal and dermal microthermal treatment zones (MTZs).
However, the precise mechanisms of dermal remodeling by Er:glass fractional laser treatment are largely unknown.
Objective: The purpose of this study was to investigate the effect of 1,550 nm Er:glass fractional laser treatment on
dermal collagen synthesis and expression of TGF-β1, a potent cytokine involved in collagen synthesis.
Methods: We treated hairless mice with several power densities (5 W 5 mJ∼20 W 20 mJ), and examined the
tissue samples on days 1, 30, and 90 after treatment. We analyzed the penetrating depth of laser treatment by
determining dermal response through assessment of type I collagen synthesis and TGF-β1 expression by H&E,
Masson-trichrome staining, western blot analysis and immunohistochemistry staining.
Results: We observed, through H&E staining, that increasing the pulse energy of fractional laser treatment correlated
with increasing depth of MTZ. Also, fractional laser treatment increased type 1 collagen synthesis on days 30 and
90, energy dependently. Immunohistochemical study showed that fractional laser treatment increased expression of
type I collagen and TGF-β1, energy dependently, with TGF-β1 expression peaking on day 1. In addition,
according to western blot analysis, expressions of TGF-β1 and type I collagen were up-regulated in an energydependent
Conclusion: Er:glass fractional laser induced dermal remodeling by up-regulation of TGF-β1 and type I collagen
synthesis, and may be a promising modality for skin rejuvenation.
- Fractional laser; TGF-β1; Type I collagen
- Appears in Collections:
- 1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
- Keimyung Author(s)
- 김성애; 이규석
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