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PCR-RFLP Analysis for Detecting Mutations at 12th Codon of Oncogene Ras in Korean Human Skin Cancer

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Author(s)
Soo Deuk KongYoung Wook RyooKyu Suk Lee
Publication Year
1998
Keyword
Codon 12 of the K-ras geneHuman skin cancer
Abstract
BACKGROUND : The mutation of ras oncogenes has been detected in a broad spectrum of human malignancy. These mutations have been localized at codons 12, 13 and 61 of three ras genes (K-,H-,N-ras). Especially, codon 12 of three ras genes are frepuently mrepuently mutated in human cancers. OBJECT : The purpose of this study is to detect mutation of codon 12 of the K-ras gene in 3 seborrheic keratoses, 6 basal cell carcinomas and 7 spuamous cell carcinomas. METHOD : We prepared chromosomal DNA from tumor specimens and perforned Polymerase Chain Reaction-Restriction Fragment Length Polymorphism analysis of codon 12 the K-ras gene. RESULT : In this study, we could not find any mutation at the codon 12 of the K-ras genes from our cases. CONCLUSION : Our results suggest that mutation of codon 12 of the K-ras gene is not common in the carcinogenesis of human skin neoplasms. It may have little relevance to the mutations of the K-ras gene. So, further studies (searching for codon 13 and 61 areas) and other techniques are needed to clarify the relationship of the K-ras gene and skin cancers in the future.
Alternative Title
한국인 피부암에서 K-Ras 암유전자의 돌연변이
Department
Dept. of Dermatology (피부과학)
Publisher
School of Medicine
Citation
대한피부연구학회지, Vol.5(1) : 97-101, 1998
Type
Article
ISSN
1225-8180
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/39896
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