나병 병변부 피부에서 Interferon-γ, Interleukin-4, Inducible Nitric Oxide Synthase 유전자 발현비교

Abstract

Leprosy presents as a clinical spectrum in which resistance correlates with a immunological reactivity, especially cell-mediated immunity. The disease provides a useful and accessible model, in this case in the skin, in which to study the dynamics of cellular immune response to an infectious pathogen, incliding the role of cytokines in those responses. To assess cellular immune response, we examined the transcripts of interferin-γ, interleukin-4 (IL-4) and inducible nitric oxide synthase (iNOS) genes in leprosy skin lesions using reverse transcription and polymerase chain reaction (RT-PCR) method. The results were as follow: 1. none of 3 examined genes were expressed in the skin of nermal control. 2. only iNOS was detected in all of 4 tuberculous leprosy skin lesions 3. only IL-4 was detected in all of 4 lepromatous leprosy skin lesions The analysis of cytokine patterns and nitric oxide effect within different inflammatory responses can provide insighs into immune mechanisms of infectious diseases and autoimmune diseases, and provide a useful framework for developing strategies for therapeutic intervention.