HL60 세포주의 분화유도에 따른 Apoptosis관련 유전자의 발현

Abstract

In hematopoietic tissues, apoptosis is coupled to terminal differentiation of myeloid progenitors. The mechanism of apoptosis associated with differentiation is poorly understood. HL60, promyelocytic leukemia cell line, was used to study expression of apoptosis related genes (bcl-2, bcl-xivs, c-iap 2，and procaspase 3) during differentiation induced by 12-O-tetradecanoylphorbol- 13-acetate (TPA). HL60 cells were differentiated to monocyte/macrophage like cells about 1 day after TPA treatment, and differentiated cells were gradually induced apoptosis from 4 days after TP A treatment. The bcl-2 mRNA and protein product were rapidly and continuously downregulated but expression of bcl-XL and c-iap 2 genes were increased in early stage of differentiation and then decreased. Expression of bcl-xs, the alternative splicing form of bcl-xL gene, was slowly and continuously increased. These data suggest that increased expression of bcl-xL and c-iap 2 genes1 may provide a mechanism through which apoptosis is blocked in early stage of HL60 cell differentiation. ᄎ韓微生物學會誌 第34卷 第1號 1999 J. Korean Soc. Microbiol,, Vol. 34，No. 1, 1999 Key Words: Differentiation, Apoptosis, HL60, bcl-2, bcl-x, c-iap 2，Procaspase 3