Cyto-Molecular Comparison Between Human Primary Cancer and its Derived Cancer Cell Line

Abstract

In order to evaluate which cyto-molecular genetic evolutionary patterns take place during the in vitro establishment of permant squamous cell carcinoma cell line of urinary bladder; cyto-molecular genetic follow up was performed on primary culture for two weeks and on cancer cell line after continued culture for three years in vitro. Near-diploid cells present on primary culture and near-hypertriploid or hypotetraploid cells; in contrast; present on cancer cell line. Chromosomal gains or losses are random from primary cancer to cancer cell line. There are two kinds of structural cytogenetic abnormalities through progress in culture time. One is maintaining abnormal clone from original cancer to derived cancer cell line. Others are cytogenetic alteration; In creasing and decreasing abnormal clones are co-existed in both group. Activations of oncogenes are different from primary cancer to cancer cell line. It is concluded that the eventual chromosomal compositions of squamous cell carcinoma of bladder derived cell line are the production of cyto-mo3ecuJar evolutions from original tumor.