Analysis of cytomegalovirus-specific T-cell responses in patients with hypertension: comparison of assay methods and antigens

Jong-Chan YounJun Yong KimMin Kyung JungHee Tae YuSu-Hyung ParkIn-Cheol KimSun Ki LeeSuk-Won ChoiSeongwoo HanKyu-Hyung RyuSungha ParkEui-Cheol Shin
Dept. of Internal Medicine (내과학)
Issue Date
Clinical Hypertension, Vol.24() : 5-5, 2018
Background: Recent studies suggest an association between cytomegalovirus (CMV) infection and hypertension. In the present study, we used a variety of antigens and different assay methods to investigate the relationship between CMV-specific T-cell responses and arterial stiffness in patients with hypertension. Methods: To evaluate arterial stiffness, pulse wave velocity (PWV) was measured in 207 hypertensive patients (average age, 63 ± 8 years). To measure CMV pp65 and IE-1-specific T-cell responses, we performed intracellular cytokine staining (ICS) and enzyme-linked immunospot (ELISPOT) assays. We also analyzed CMV-specific T-cell responses against 10 different CMV antigens using ELISPOT assays. Results: In patients with hypertension, senescent CD8+ T-cell frequencies were significantly correlated with arterial stiffness. Moreover, arterial stiffness was independently associated with CMV pp65-specific CD8+ T-cell responses as measured by ICS. CMV-specific CD8+ T-cell responses measured by ICS and ELISPOT assays showed good agreement and significant correlation with each other. ELISPOT analyses against 10 different CMV antigens revealed a consistent response pattern irrespective of age, gender, and diabetes. Conclusions: CMV pp65-specific CD8+ T-cell responses were independently correlated with arterial stiffness in patients with hypertension. Additionally, the results of ICS and ELISPOT assays showed a significant correlation and good agreement with each other. These findings are important for guiding choices regarding the broad clinical application of CMV-specific T-cell response assays in this patient population.
Arterial stiffnessCytomegalovirusEnzyme-linked immunospot (ELISPOT) assayIntracellular cytokine staining (ICS)T cell
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