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dc.contributor.authorSeung-Eun Song-
dc.contributor.authorSu-Kyung Shin-
dc.contributor.authorHyun-Woo Cho-
dc.contributor.authorSeung-Soon Im-
dc.contributor.authorJae-Hoon Bae-
dc.contributor.authorSeon Min Woo-
dc.contributor.authorTaeg-Kyu Kwon-
dc.contributor.authorDae-Kyu Song-
dc.date.accessioned2019-03-11T16:37:27Z-
dc.date.available2019-03-11T16:37:27Z-
dc.date.issued2018-
dc.identifier.citationMolecular and Cellular Biochemistry, Vol.444(1-2) : 17-25, 2018-
dc.identifier.issn0300-8177-
dc.identifier.otheroak-2018-1182-
dc.identifier.urihttp://kumel.medlib.dsmc.or.kr/handle/2015.oak/41552-
dc.description.abstractIn this study, we examined the effect of tomatidine on tumor necrosis factor (TNF)-α-induced apoptosis in C2C12 myoblasts. TNF-α treatment increased cleaved caspase 3 and cleaved poly (ADP-ribose) polymerase (PARP) protein levels in a dose- and time-dependent manner. Pretreatment of cells with 10 μM tomatidine prevented TNF-α-induced apoptosis, caspase 3 cleavage, and PARP cleavage. Cells were treated with 100 ng/mL TNF-α for 24 h, and flow cytometry was utilized to assess apoptosis using annexin-V and 7-aminoactinomycin D. TNF-α up-regulated activating transcription factor 4 (ATF4) and C/EBP homologous protein (CHOP) expression. This effect was suppressed by pretreatment with tomatidine. Pretreatment with 4-phenylbutyric acid (a chemical chaperone) also inhibited TNF-α-induced cleavage of caspase 3 and PARP and up-regulation of ATF4 and CHOP expression. In addition, tomatidine-mediated inhibition of phosphorylation of c-Jun amino terminal kinase (JNK) attenuated TNF-α-induced cleavage of PARP and caspase 3. However, tomatidine did not affect NF-κB activation in TNF-α-treated C2C12 myoblast cells. Taken together, the present study demonstrates that tomatidine attenuates TNF-α-induced apoptosis through down-regulation of CHOP expression and inhibition of JNK activation.-
dc.description.statementofresponsibilityrestriction-
dc.publisherSchool of Medicine (의과대학)-
dc.rightsBY_NC_ND-
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/2.0/kr-
dc.subjectTomatidine-
dc.subjectTNF-α-
dc.subjectC2C12 myoblast-
dc.subjectER stress-
dc.subjectApoptosis-
dc.titleTomatidine inhibits tumor necrosis factor-α-induced apoptosis in C2C12 myoblasts via ameliorating endoplasmic reticulum stress-
dc.typeArticle-
dc.contributor.localauthor송대규-
dc.contributor.localauthor임승순-
dc.contributor.localauthor배재훈-
dc.contributor.localauthor권택규-
dc.contributor.alternativelocalauthorSong, Dae Kyu-
dc.contributor.alternativelocalauthorIm, Seung Soon-
dc.contributor.alternativelocalauthorBae, Jae Hoon-
dc.contributor.alternativelocalauthorKwon, Taeg Kyu-
dc.contributor.departmentDept. of Physiology (생리학)-
dc.contributor.departmentDept. of Immunology (면역학)-
dc.citation.volume444-
dc.citation.number1-2-
dc.citation.startpage17-
dc.citation.titleMolecular and Cellular Biochemistry-
dc.citation.endpage25-
dc.identifier.doi10.1007/s11010-017-3226-3-
dc.identifier.urlhttps://link.springer.com/article/10.1007%2Fs11010-017-3226-3-


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