계명대학교 의학도서관 Repository

Mito-TEMPO improves development competence by reducing superoxide in preimplantation porcine embryos

Metadata Downloads
Affiliated Author(s)
이윤한서지혜
Alternative Author(s)
Lee, Yun HanSeo, Ji Hye
Journal Title
Scientific Reports
ISSN
2045-2322
Issued Date
2018
Abstract
Mito-TEMPO is a well-known mitochondria-specific superoxide scavenger. However, the effect of Mito-TEMPO on porcine embryo development, to our knowledge, has not been studied yet. In the present study, porcine embryos were classified into two groups (G1 and G2) based on the cytoplasm lipid contents at the zygote stage. The development of blastocysts derived from G2 zygotes was reduced (G2:16.2 ± 7.9% vs G1: 26.5 ± 5.9%; 1.6-fold, p < 0.05) compared to those from G1 zygotes. In G2 embryos, the proportion of TUNEL-positive cells was also higher than that of G1 embryos. Superoxide in G2 embryos was significantly increased compared to that in G1 embryos. Mitochondrial membrane potential and ATP production were lower in G2 embryos than in G1 embryos. Phosphorylation of Drp1 at Ser 616 increased in G1 embryos during the cleavage stages compared to that in the zygote but was not significantly different in G2 embryos. Then, the effects of Mito-TEMPO were investigated in G2 embryos. Blastocyst formation rate (G2: 19.1 ± 5.1% vs G2 + Mito-TEMPO: 28.8 ± 4.0%; 1.5-fold, p < 0.05) and mitochondrial aggregation were recovered after superoxide reduction by Mito-TEMPO treatment. Thus, we showed that Mito-TEMPO improves blastocyst development by superoxide reduction in porcine embryos in vitro.
Department
Dept. of Molecular Medicine (분자의학)
Dept. of Biochemistry (생화학)
Publisher
School of Medicine (의과대학)
Citation
Seul-Gi Yang et al. (2018). Mito-TEMPO improves development competence by reducing superoxide in preimplantation porcine embryos. Scientific Reports, 8(1), 10130–10130. doi: 10.1038/s41598-018-28497-5
Type
Article
ISSN
2045-2322
DOI
10.1038/s41598-018-28497-5
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/41731
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
공개 및 라이선스
  • 공개 구분공개
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.