계명대학교 의학도서관 Repository

An affinity peptide-incorporated electrochemical biosensor for the detection of neutrophil gelatinase-associated lipocalin

Metadata Downloads
Affiliated Author(s)
송대규
Alternative Author(s)
Song, Dae Kyu
Journal Title
Biosensors and Bioelectronics
ISSN
1873-4235
Issued Date
2019
Keyword
Neutrophil gelatinase-associated lipocalinPeptide receptorSensitivityElectrochemical sensor
Abstract
In this study, we demonstrate a novel affinity peptide-incorporated electrochemical biosensor for the detection of acute kidney injury and the diabetic biomarker neutrophil gelatinase-associated lipocalin (NGAL). Biopanning of the M13 phage display library over immobilized NGAL led to the rapid identification of unique affinity peptide with an amino acid sequence of DRWVARDPASIF, and the peptide-displayed phage particles were found to be specific affinities for NGAL. To address the development of peptide-based electrochemical sensor, a series of synthetic peptides away from phage particles was rationally designed, chemically synthesized, and immobilized to a gold sensor layer. Among five synthetic peptide derivatives tested, NGAL BP1 was selected as most promising recognition receptor, and its binding affinity was monitored by SWV and EIS. Using EIS, the limit of detection (LOD) was 1.74 ng/mL, while SWV had a LOD of 3.93 ng/mL. The detection performance of the peptide-incorporated sensor was comparable to commercially available ELISA NGAL detection kits. In addition, the validation of the peptide sensor was also confirmed with plasma from patients, and it was observed that the sensitivity of the peptide sensor showed a statistically significant difference. Our results show that the phage and peptide sensor system could detect NGAL with high sensitivity and selectivity, and this suggests its potential use as a biosensing platform for monitoring NGAL in a miniaturized electrochemical biosensor.
Department
Dept. of Physiology (생리학)
Publisher
School of Medicine (의과대학)
Citation
Chae Hwan Cho et al. (2019). An affinity peptide-incorporated electrochemical biosensor for the detection of neutrophil gelatinase-associated lipocalin. Biosensors and Bioelectronics, 142. doi: 10.1016/j.bios.2019.111482
Type
Article
ISSN
1873-4235
DOI
10.1016/j.bios.2019.111482
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/42050
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
공개 및 라이선스
  • 공개 구분공개
  • 엠바고Forever
파일 목록
  • 관련 파일이 존재하지 않습니다.

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.