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Impact of calcineurin inhibitors on rat glioma cells viability

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Affiliated Author(s)
박우영백진혁박성배한승엽문교철진규복
Alternative Author(s)
Park, Woo YoungPaek, Jin HyukPark, Sung BaeHan, Seung YeupMun, Kyo CheolJin, Kyu Bok
Journal Title
Yeungnam University Journal of Medicine
ISSN
2384-0293
Issued Date
2019
Keyword
Calcineurin inhibitorsGlioma cellKidney transplantationNeurotoxicity
Abstract
Background:
Although kidney transplantation outcomes have improved dramatically after using calcineurin inhibitors (CNIs), CNI toxicity continues to be reported and the mechanism remains uncertain. Here, we investigated the neurotoxicity of CNIs by focusing on the viability of glioma cells.

Methods:
Glioma cells were treated with several concentrations of CNIs for 24 hours at 37°C and their cell viability was evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay.

Results:
Exposure to 0, 0.25, 0.5, 2.5, 5.0, and 10.0 mM concentrations respectively showed 100%, 64.3%, 61.3%, 68.1%, 62.4%, and 68.6% cell viability for cyclosporine and 100%, 38.6%, 40.8%, 43.7%, 37.8%, and 43.0% for tacrolimus. The direct toxic effect of tacrolimus on glioma cell viability was stronger than that of cyclosporine at the same concentration.

Conclusion:
CNIs can cause neurological side effects by directly exerting cytotoxic effects on brain cells. Therefore, we should carefully monitor the neurologic symptoms and level of CNIs in kidney transplant patients.
Department
Dept. of Internal Medicine (내과학)
Dept. of Biochemistry (생화학)
Publisher
School of Medicine (의과대학)
Citation
Jeong Hun Seong et al. (2019). Impact of calcineurin inhibitors on rat glioma cells viability. Yeungnam University Journal of Medicine, 36(2), 105–108. doi: 10.12701/yujm.2019.00108
Type
Article
ISSN
2384-0293
DOI
10.12701/yujm.2019.00108
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/42187
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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