계명대학교 의학도서관 Repository

Isocitrate dehydrogenase 2 protects mice from high-fat diet-induced metabolic stress by limiting oxidative damage to the mitochondria from brown adipose tissue

Metadata Downloads
Affiliated Author(s)
이재호권택규배재훈송대규송민호임승순
Alternative Author(s)
Lee, Jae HoKwon, Taeg KyuBae, Jae HoonSong, Dae KyuIm, Seung Soon
Journal Title
Experimental and Molecular Medicine
ISSN
2092-6413
Issued Date
2020
Abstract
Isocitrate dehydrogenase 2 (IDH2) is an NADP+-dependent enzyme that catalyzes the oxidative decarboxylation of isocitrate to α-ketoglutarate in the mitochondrial matrix, and is critical for the production of NADPH to limit the accumulation of mitochondrial reactive oxygen species (ROS). Here, we showed that high-fat diet (HFD) feeding resulted in accelerated weight gain in the IDH2KO mice due to a reduction in whole-body energy expenditure. Moreover, the levels of NADP+, NADPH, NAD+, and NADH were significantly decreased in the brown adipose tissue (BAT) of the HFD-fed IDH2KO animals, accompanied by decreased mitochondrial function and reduced expression of key genes involved in mitochondrial biogenesis, energy expenditure, and ROS resolution. Interestingly, these changes were partially reversed when the antioxidant butylated hydroxyanisole was added to the HFD. These observations reveal a crucial role for IDH2 in limiting ROS-dependent mitochondrial damage when BAT metabolism is normally enhanced to limit weight gain in response to dietary caloric overload.
Department
Dept. of Anatomy (해부학)
Dept. of Immunology (면역학)
Dept. of Physiology (생리학)
Publisher
School of Medicine (의과대학)
Citation
Jae-Ho Lee et al. (2020). Isocitrate dehydrogenase 2 protects mice from high-fat diet-induced metabolic stress by limiting oxidative damage to the mitochondria from brown adipose tissue. Experimental and Molecular Medicine, 52(2), 238–252. doi: 10.1038/s12276-020-0379-z
Type
Article
ISSN
2092-6413
DOI
10.1038/s12276-020-0379-z
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/43075
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
공개 및 라이선스
  • 공개 구분공개
파일 목록

Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.