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Optimized culture systems for the preimplantation ICR mouse embryos with wide range of EDTA concentrations

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Affiliated Author(s)
허윤석
Alternative Author(s)
Heo, Yun Seok
Journal Title
R Soc Open Sci
ISSN
2054-5703
Issued Date
2021
Abstract
In this study, in vitro preimplantation embryo culture media especially for outbred stock mice (Institute of Cancer Research (ICR)) were optimized with different concentrations of ethylenediaminetetraacetic acid (EDTA). A plot with embryo development rates against EDTA concentrations ranging from 0 to 500 µM showed a unique pattern with two characteristic peaks. Two hundred micromolar was adopted as an optimal concentration of EDTA. The optimized media were also evaluated with two culture systems: conventional large volume culture system (1 ml) and micro-droplet culture system. In the conventional large volume culture system, the blastocyst development rates were compared among three different media (F-10, KSOM and KSOM with the optimized 200 µM EDTA). The rates were 0.4%, 16.7% and 57.6%, respectively. The development rates for the micro-droplet (10 µl) culture system were 73.9%. In conclusion, 200 µM EDTA concentration in 10 µl droplets in the KSOM medium was found as the most suitable culture conditions for ICR mouse embryos, as the blastocyst development rate was higher in the micro-droplet culture system than in the traditional conventional large volume culture system.
Department
Dept. of Biomedical Engineering (의용공학과)
Publisher
School of Medicine (의과대학)
Citation
Seema Thapa et al. (2021). Optimized culture systems for the preimplantation ICR mouse embryos with wide range of EDTA concentrations. R Soc Open Sci, 8(4), 201752. doi: 10.1098/rsos.201752
Type
Article
ISSN
2054-5703
DOI
10.1098/rsos.201752
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/43760
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biomedical Engineering (의용공학과)
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