Identification of Pre-Diabetic Biomarkers in the Progression of Diabetes Mellitus
- Affiliated Author(s)
- 배재훈; 한유진; 송대규; 권택규; 임승순
- Alternative Author(s)
- Bae, Jae Hoon; Han, Eu Gene; Song, Dae Kyu; Kwon, Taeg Kyu; Im, Seung Soon
- Journal Title
- Biomedicines
- ISSN
- 2227-9059
- Issued Date
- 2022
- Keyword
- diabetes mellitus; type 2 diabetes mellitus; pre-diabetes; biomarkers; metabolic disease
- Abstract
- Type 2 diabetes mellitus (T2DM) is a major global health issue. The development of T2DM is gradual and preceded by the pre-diabetes mellitus (pre-DM) stage, which often remains undiagnosed. This study aimed to identify novel pre-DM biomarkers in a high-fat diet (HFD)-induced pre-DM mouse model. Male C57BL/6J mice were fed either a chow diet or HFD for 12 weeks. Serum and liver samples were isolated in a time-dependent manner. Semi-quantitative assessment of secretory cytokines was performed by cytokine array analysis, and 13 cytokines were selected for further analysis based on the changes in expression levels in the pre-DM and T2DM stages. HFD-fed mice gained body weight and exhibited high serum lipid, liver enzyme, glucose, and insulin levels during the progression of pre-DM to T2DM. The mRNA expression of inflammatory and lipogenic genes was elevated in HFD-fed mice The mRNA expression of Fc receptor, IgG, low affinity Iib, lectin, galactose binding, soluble 1, vascular cell adhesion molecule 1, insulin-like growth factor binding protein 5, and growth arrest specific 6 was elevated in the pre-DM, which was confirmed by measuring protein levels. Our study identified novel pre-DM biomarkers that may help to delay or prevent the progression of T2DM.
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