https://kumel.medlib.dsmc.or.kr/handle/2015.oak/29775 Sat, 04 Apr 2026 17:17:33 GMT 2026-04-04T17:17:33Z https://kumel.medlib.dsmc.or.kr/handle/2015.oak/45584 Title: Association of sleep quality and mitochondrial DNA copy number in healthy middle-aged adults Author(s): Seolbin Han; Dae-Kwang Kim; Sang-Eun Jun; Nahyun Kim Abstract: Objectives: Mitochondria contribute to various compromised health, yet the association between sleep and mitochondria remains unclear. This study investigated the association between sleep quality and mitochondrial function in healthy middle-aged adults in the Republic of Korea. Method: This cross-sectional study recruited 238 middle-aged adults using convenience sampling. Sleep quality was assessed using the Pittsburgh Sleep Quality Index (PSQI). Mitochondrial function, represented by mitochondrial DNA copy number (mtDNAcn), was measured using real-time quantitative polymerase chain reaction on peripheral blood leukocytes. Multivariate linear regression analyses were performed to determine the association between sleep quality and mtDNAcn. Results: Sleep quality was negatively associated with mtDNAcn (r = -.15, p = .025); the poor sleep quality group had a notably lower mtDNAcn compared to the good sleep quality group (t = 2.40, p = .017). Among the PSQI components, sleep latency was significantly associated with reduced mtDNAcn (r = -.18, p = .005). Univariate regression analysis revealed that mtDNAcn was significantly associated with education level (β = 0.15, p = .017), shift work (β = -0.17, p = .010), global PSQI score (β = -0.15, p = .025), and sleep latency (β = -0.18, p = .005). After adjusting for educational level and shift work in the final model, longer sleep latency was independently associated with reduced mtDNAcn (β = -.16, p = .011). Conclusions: Poor sleep quality is associated with reduced mtDNAcn, suggesting a potential biological mechanism whereby poor sleep quality, specifically long sleep latency, accelerates cellular aging and impairs health through mitochondrial dysfunction. These findings enhance our understanding of the health effects of sleep quality and highlight the importance of screening and intervention strategies for mitochondrial dysfunction. Sun, 31 Dec 2023 15:00:00 GMT https://kumel.medlib.dsmc.or.kr/handle/2015.oak/45584 2023-12-31T15:00:00Z https://kumel.medlib.dsmc.or.kr/handle/2015.oak/44821 Title: 성인간호연구에서의 생물표지자 ‘텔로미어 길이’의 이해와 활용: 종설연구 Author(s): Seolbin Han; Jihee Min; Dae-Kwang Kim; In Deok Kong; Nahyun Kim Abstract: Purpose: This study aimed to provide an overview of telomere length (TL) as an emerging biomarker in adult healthcare. Additionally, some measurement considerations and future directions for its application in adult nursing research were described. Methods: A comprehensive literature review was conducted. Results: TL is a widely known indicator of aging and aging-related diseases at the molecular level. Throughout the literature, TL has been established as a useful biomarker that is indicative of aging-related diseases such as cancer, metabolic diseases, and psychological distress and their resulting health conditions. The main pathway of TL shortening appears as an interaction between genetic and environmental factors through a mechanism commonly known as oxidative stress and inflammation. TL attrition may be slowed down, stopped, or even lengthened by interventions such as mindfulness, meditation, exercise, lifestyle modifications, and cognitive behavioral therapy, which have been demonstrated to have a positive effect on TL. As these interventions have been widely applied in adult nursing research, the value and scope of adult nursing science can be expanded by using TL in such research. Conclusion: TL has been shown to be associated with age-related diseases, which are mainly studied in adult nursing research. Therefore, it is necessary to explore various nursing phenomena using TL as a biomarker through adult nursing research and to develop nursing interventions that have a positive effect on TL. Sat, 31 Dec 2022 15:00:00 GMT https://kumel.medlib.dsmc.or.kr/handle/2015.oak/44821 2022-12-31T15:00:00Z https://kumel.medlib.dsmc.or.kr/handle/2015.oak/44685 Title: Analysis of microsatellite instability in Korean patients with pancreatic cancer Author(s): Mohammad R. ALAM; Yong H. KIM; Alaa ALHAZMI; Shafiul HAQUE; Yoo N. KANG; Hye R. JUNG; Mi-Yeung SOHN; Dae-Kwang KIM Abstract: BACKGROUND: Pancreatic cancer (PC) is a dangerous malignancy with a high mortality rate. Diagnosing PC at an early stage is difficult, and approximately 5 % of the patients survive for 5 years. Microsatellite instability (MSI) plays an important role in colorectal cancer (CRC) for prognosis and immunotherapy. Evaluation of MSI status is important as it is recognized biomarker for the positive response of immune checkpoint blockade therapy in cancer. To our knowledge, there is no report yet on the prevalence of MSI in Korean PC patients. Studies have reported conflicting prevalence of MSI in PC. METHODS: Therefore, to improve the likelihood of MSI identification in PC, we included 133 patients with PC; paired tumor and normal tissue DNA were isolated and MSI was analyzed using Promega panel and immunohistochemistry (IHC) was also performed. RESULTS: Our results from the Promega panel indicated that one (0.7 %) tumor was MSI-high (MSI-H), 13 (9.8 %) were MSI-low (MSI-L), and 119 (89.5 %) were microsatellite stable (MSS). IHC result also confirmed dMMR in only one sample. CONCLUSIONS: The finding of low incidence of MSI-H observed by the Promega panel also matched IHC results, so this study suggested that in Korean PC patients, MSI prevalence is infrequent. Fri, 31 Dec 2021 15:00:00 GMT https://kumel.medlib.dsmc.or.kr/handle/2015.oak/44685 2021-12-31T15:00:00Z https://kumel.medlib.dsmc.or.kr/handle/2015.oak/43782 Title: Differences in the mitochondrial microsatellite instability of Keratoacanthoma and cutaneous squamous cell carcinoma Author(s): Mohammad Rizwan Alam; Ahmad Alsulimani; Shafiul Haque; Hye Ra Jung; Jae-Ho Lee; Chang-Ho Jeon; Dae-Kwang Kim Abstract: Keratoacanthoma (KA) is a common cutaneous neoplasm which often resembles typical squamous cell carcinoma (SCC) in both its clinical and historical presentation. Several studies have attempted to identify methods for distinguishing between KA and SCC, however, none of these have proven to play any obvious roles in these tumors. Given this we went on to evaluate mitochondrial microsatellite instability (mtMSI) in KA and SCC in an effort to understand these tumors better. DNA was isolated from paired normal and tumoral tissues donated by 57 KA patients and 43 SCC patients. MtMSI was then analyzed using eight microsatellite markers and was observed in 2 (3.5%) of the 57 KA patients and 8 (18.6%) of the 43 SCC patients, respectively. MtMSI was also shown to affect different locations depending on tumor type. In KA patients, mtMSI was detected at mitochondrial D514 D-loop and presented with (CA) n repeats, in contrast, all of the SCC patient experienced mtMSI at the D310 with (C)n repeats of the D-loop region. These differences in location were found to be significant, which may support the hypothesis that KA and SCC have different pathogenetic pathways. Our results also suggest that mtMSI may be a candidate for developing novel differential diagnostic methods for KA and SCC. Thu, 31 Dec 2020 15:00:00 GMT https://kumel.medlib.dsmc.or.kr/handle/2015.oak/43782 2020-12-31T15:00:00Z