한국산 가토에서 얻어진 보체의 인간조직적합성 항원시험에의 적용

Abstract

This study was carried out to determine the usability of Korean albino rabbit serum as a complement in the test of HLA. Thirty albino male rabbits, weighing 2.5kg were purchased from the market. They were kept in an outdoor rabbit kennel and were fed ordinary diet for 30 days. Each was fasted overnight prior to bleeding of 20ml from ear vein. The blood samples were clotted separately to avoid hemolysis. The serum was centrifuged at 4°C for removal of residual RBC and stored at —70°C. No special treatment was given during and handling. Fifteen rabbits of negative antihuman factors tested by microcytotoxicity test were selected for this experiment. Average CH50 of the 15 rabbits was 0.29. Among these 15 rabbits serum, 13 sample showed positive at the dilution of 1:1 and 1 ： 2 through the microcytotoxicity test of Terasaki with ABC tray. Two rabbit serum showed availability as a complement for DR tray. In accordance with this result, it was suggested that Korean albino rabbit serum could be used as a complement for HLA test.