B 세포융합과 세포선별 방법

Other Titles
Technique on the B Cell Selection Following Fused Cells.
Authors
장성익최인장
Department
Dept. of Anatomy (해부학)
Issue Date
1985
Citation
Keimyung Medical Journal, Vol.4(2) : 138-142, 1985
Abstract
For the the basic technique of the monoclonal antibody, cell fusion was performed with spleen cell of BALB/C mouse and myeloma cell line irealed with 41^ PEG, 45% PEG and 50% PEG, and cell selection was performed with exchange of HAT media only and HAT contained RPMI plus 15% FCS for one week respectativdy. The results were summeri^ed as follows; 1. The number of chromosome on lymphocyte of BALB/C mouse was 40 and in the P3-UL cell line it was variable from 61 Lo 64 numbers. 2. There was a strong tendency for loss <)f chromusumfs in f니、ed cells as a range 84 to 94 chromosomal numbers. 3. For the cell fusion, \vc found it was the best stai li^ (reaied with 50% PEG and for the cell selection, it was better condition used to HAT and RPMI plus 15% FCS than used to HAT only.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/14387
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
2. Keimyung Medical Journal (계명의대 학술지) > 1985
Keimyung Author(s)
장성익; 최인장
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