B 세포융합과 세포선별 방법

Abstract

For the the basic technique of the monoclonal antibody, cell fusion was performed with spleen cell of BALB/C mouse and myeloma cell line irealed with 41^ PEG, 45% PEG and 50% PEG, and cell selection was performed with exchange of HAT media only and HAT contained RPMI plus 15% FCS for one week respectativdy.

The results were summeri^ed as follows；

1. The number of chromosome on lymphocyte of BALB/C mouse was 40 and in the P3-UL cell line it was variable from 61 Lo 64 numbers.

2. There was a strong tendency for loss <)f chromusumfs in f니、ed cells as a range 84 to 94 chromosomal numbers.

3. For the cell fusion, \vc found it was the best stai li^ (reaied with 50% PEG and for the cell selection, it was better condition used to HAT and RPMI plus 15% FCS than used to HAT only.