Three combined mechanical and enzymatic disaggregation techniques and a simple mechanica] disaggregation procedure were compared. The combined procedures involved a mechanical comminution the tumour tissue followed by incubation in trypsin. In one method, the tissue was subjected to log-term trypsinization at 4°C, and in the other procedure, repeated short-term trypsinization at 37° C, and in the third method, trypsinization at 4°C and collaganase procedure was applied: The results were compared in terms of the yield of viable cells. The combined techniques provided reproducible cell yields of 2.8-5.8X107 viable cells g"1 of tissue, whereas only a small number of tumour cells was produced by the mechanical method. Especially, after trypsinization at 4°C and collagenase procedure, we gained high cell yield of 5.4—5.8X107 viable cells g-1 of tissue.
We introduce this advanced technique on tumour cell to detact the prognosis of patients suffered from neoplasm clinically.