암세포 염색체 연구에 있어서 새로운 방법

Other Titles
Advanced technique on chromosome study of tumour cells
Authors
장성익최인장이인환
Department
Dept. of Anatomy (해부학)
Issue Date
1986
Citation
Keimyung Medical Journal, Vol.5(1) : 22-25, 1986
Abstract
Three combined mechanical and enzymatic disaggregation techniques and a simple mechanica] disaggregation procedure were compared. The combined procedures involved a mechanical comminution the tumour tissue followed by incubation in trypsin. In one method, the tissue was subjected to log-term trypsinization at 4°C, and in the other procedure, repeated short-term trypsinization at 37° C, and in the third method, trypsinization at 4°C and collaganase procedure was applied: The results were compared in terms of the yield of viable cells. The combined techniques provided reproducible cell yields of 2.8-5.8X107 viable cells g"1 of tissue, whereas only a small number of tumour cells was produced by the mechanical method. Especially, after trypsinization at 4°C and collagenase procedure, we gained high cell yield of 5.4—5.8X107 viable cells g-1 of tissue. We introduce this advanced technique on tumour cell to detact the prognosis of patients suffered from neoplasm clinically.
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/14416
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
2. Keimyung Medical Journal (계명의대 학술지) > 1986
Keimyung Author(s)
장성익; 최인장; 이인환
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