흰쥐간 세포분획법 1. Mitochondria 및 Microsome의 분리

Abstract

A procedure for the isolation of highly purified mitochondria and microsomes from normal rat liver is described.

The liver was homogenized in 0.25M sucrose and mitochondrial fraction was prepared by 20 to 45% sucrose linear density gradient centrifugation. Microsomal fraction was also prepared by 10 to 35% sucrose linear density gradient centrifugation.

The purity of mitochondrial and microsomal fractions confirmed by morphometry showed about 92 and 87% respectively.

Monoamine oxidase activity was found in mitochondrial and microsomal fractions which contain about 64 and 17% of the total monoamine oxidase activity respectively. About 71% of the total glucose-6-phosphatase activity was found in the microsomal fraction, And alcohol dehydrogenase activity was only found in the cytosol fraction.