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흰쥐간 세포분획법 1. Mitochondria 및 Microsome의 분리

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Author(s)
곽정식곽춘식
Keimyung Author(s)
Kwak, Chun Sik
Department
Dept. of Biochemistry (생화학)
Journal Title
Keimyung Medical Journal
Issued Date
1986
Volume
5
Issue
1
Abstract
A procedure for the isolation of highly purified mitochondria and microsomes from normal rat liver is described.

The liver was homogenized in 0.25M sucrose and mitochondrial fraction was prepared by 20 to 45% sucrose linear density gradient centrifugation. Microsomal fraction was also prepared by 10 to 35% sucrose linear density gradient centrifugation.

The purity of mitochondrial and microsomal fractions confirmed by morphometry showed about 92 and 87% respectively.

Monoamine oxidase activity was found in mitochondrial and microsomal fractions which contain about 64 and 17% of the total monoamine oxidase activity respectively. About 71% of the total glucose-6-phosphatase activity was found in the microsomal fraction, And alcohol dehydrogenase activity was only found in the cytosol fraction.
Alternative Title
Cell Fractionation Method of the Rat Liver

1. Isolations of Mitochondria and Microsomes
Keimyung Author(s)(Kor)
곽춘식
Publisher
Keimyung University School of Medicine
Citation
곽정식 and 곽춘식. (1986). 흰쥐간 세포분획법 1. Mitochondria 및 Microsome의 분리. Keimyung Medical Journal, 5(1), 45–53.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/14420
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 1986
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
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