유암조직에서 단세포군항체를 이용한 면역세포화학법에 의한 에스트로겐 수용체의 측정

Abstract

Estrogen receptor(ER) is an unique protein that specifically binds estrogens in tissue that responds to this hormone. In order for tissue to recognize estrogens, it is considered necessary for ER to be present. Receptor assay can be used in prediction of response of hormonal therapy in treatment of breast cancers. Immunocytochemical assays(ICA) involve localization and staining of ER in tissues or cell preparations using monoclonal antibodies. Frozen sections from 13 human breast carcinomas were examined for the presence and distribution of ER-containing tumor cells using Abbott ER-ICA monoclonal antibodies and immunoperoxidase technique. Estrogen receptor was localized only in the nuclei in breast tumor cells in 6 cases. Serveral staining patterns were observed. Thus, an immunocytochemical assay for estrogen receptor may provide additional information for identifying hormonally responsive breast cancers.