계명대학교 의학도서관 Repository

피부 섬유아세포에서의 교원질 및 Fibronectin 유전자의 발현

Metadata Downloads
Author(s)
이규석
Alternative Author(s)
Lee, Kyu Suk
Publication Year
1991
Keyword
CollagenFibronectinGene expression
Abstract
The expression of various extracellular matrix genes is examined by skin fivroblast cultures and placental tissues using Northern and slot-blot hybridization. Total RNA from 3-6 passaged fibrobasts cultures was isolated.
In Northern blot analysis, type Ⅰ and type Ⅲprocollagen-specific mRNA transcripts from cultured skin fibroblasts demostrated the specificity of hybridization with pro α1(Ⅰ) and pro α1(Ⅲ) collagen. specifically, pro α1(Ⅰ) or pro α1(Ⅲ) collagen cDNA probes hybridized to two distinct mRNA species with apparen sizes of 5.8 and 4.8 kilobases(kb), or 5.4 and 4.8 kb, respectively. The molecular size of fibronectin and β-actin mRNA revealed 8.0 kb and 2.0 kb, respectively. Therefore, it showed no changes of sizes of mRNAs between skin fibroblasts and placental tissues.
In slot-bolt analysis, the levels of type Ⅰ,Ⅲ collagen and fibronectin mRNA in skin fibroblasts is higher each 18.8, 3.2, and 1.7 folds than in placental tissues. And determination of type Ⅰ/Ⅲ procollagen ratios revealed 4.4in fibroblast cultures, and 0.75 in placental tissues.
This results indicate that type Ⅰ collgaen is predominant extracelluar matrix produced by skin fibroblasts when compaired to placental tissues.
Alternative Title
Expression of Type I, III Collagen and Fibronectin Genes by Skin Fibroblasts
Department
Dept. of Dermatology (피부과학)
Publisher
Keimyung University School of Medicine
Citation
Keimyung Medical Journal, Vol.10(3) : 387-394, 1991
Type
Article
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/14702
Authorize & License
  • AuthorizeOpen
Files in This Item:

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.