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배양된 섬유아세포에서 탄력소 mRNA의 분석

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Author(s)
류영욱이규석
Alternative Author(s)
Ryoo, Young WookLee, Kyu Suk
Issued Date
1993
Keyword
ElastinGene expression
Abstract
The expression of elastin gene is examined by skin fibroblast cultures and placental tissues using Northern and slot-blot hybridization. Total RNA from 3-6 passaged fibroblasts cultures was isolated.
In Northern blot analysis, elastin, fibronectin and β-actin mRNA transcripts from cultured keloid and normal skin fibroblast and placenta, demonstrated the specificity of hybridization with elastin, frbronectin and β-actinl. Elastin cDNA prlbe hybridized mRNA with sizes of 3.5kb. The molecular size of fibronectin and β-actin mRNA revealed 8.0kb and 2.0kb, respectively. Therefore, it showed no difference of size of mRNAs among keloid and normal skin fibroblst and placenta tissues.
In slot blot analysis, the levels of elastin in keloid is higher 1.46 fold than control skin fibroblast. And determination of elastin/type I collagen ratio revealed 0.067 in control skin fibroblast and 0.031 in keloid.
This results indicate that elastin gene expression in keloid is increased compared to control skin fibroblst and placenta.
Alternative Title
Analysis of Elastin mRNA in Skin Fibroblast Cultures
Department
Dept. of Dermatology (피부과학)
Publisher
Keimyung University School of Medicine
Citation
Keimyung Medical Journal, Vol.12(3) : 345-350, 1993
Type
Article
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/14828
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 1993
1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
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