배양된 섬유아세포에서 탄력소 mRNA의 분석

Other Titles
Analysis of Elastin mRNA in Skin Fibroblast Cultures
Authors
류영욱이규석
Department
Dept. of Dermatology (피부과학)
Issue Date
1993
Citation
Keimyung Medical Journal, Vol.12(3) : 345-350, 1993
Abstract
The expression of elastin gene is examined by skin fibroblast cultures and placental tissues using Northern and slot-blot hybridization. Total RNA from 3-6 passaged fibroblasts cultures was isolated. In Northern blot analysis, elastin, fibronectin and β-actin mRNA transcripts from cultured keloid and normal skin fibroblast and placenta, demonstrated the specificity of hybridization with elastin, frbronectin and β-actinl. Elastin cDNA prlbe hybridized mRNA with sizes of 3.5kb. The molecular size of fibronectin and β-actin mRNA revealed 8.0kb and 2.0kb, respectively. Therefore, it showed no difference of size of mRNAs among keloid and normal skin fibroblst and placenta tissues. In slot blot analysis, the levels of elastin in keloid is higher 1.46 fold than control skin fibroblast. And determination of elastin/type I collagen ratio revealed 0.067 in control skin fibroblast and 0.031 in keloid. This results indicate that elastin gene expression in keloid is increased compared to control skin fibroblst and placenta.
Keywords
ElastinGene expression
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/14828
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 1993
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Dermatology (피부과학)
Keimyung Author(s)
류영욱; 이규석
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