표피 종양에서 Epidermal Growth Factor Receptor의 발현비교

Abstract

Epidermal growth factor(EGF) and transforming growth factor alpha(TGFα) are important keratinocyte mitogens. Their effect are mediated by a cell membrane receptor(EGFR), quantitive and qualitive abnormalities of which may be responsible for deranged keratinocyte proliferation and differentiation. We have therefore examined EGFR expression immunohistochemically in a variety of benign and malignant epithelial neoplasms using monoclonal antibodies to the extracellular and intracellular receptor domains, In benign tumor(seborrheic keratosis), there was an ordered pattern of EGFR expression. In malignant tumors(basal and squamous cell carcinoma), there was loss of membrane labelling and cytoplasmic accumulation of the receptor. In premalignant prolifertation(Bowen's disease), there was loss of membrane receptor with absent cytoplasmic EGFR. In slot-blot analysis, the levels of EGFR mRNA in sguamous cell carcinoma and basal cell carcinoma revealed 3 fold elevation when compared to normal skin. We suggest that dysregulation of the EGFR may be important in the development of cutaneous epithelial malignancies.