B형 간염바이러스 유전자 및 유전자 이입 항원제공세포를 이용한 항바이러스 항체생성 유도

Abstract

In order to develop effective vaccine to recover incomplete immunity in patients with chronic hepatitis B virus infection, we produced various types of vaccine for Hepatitis B virus core (HBc) antigen and compared their effects on anti-HBc antibody response. Intramuscular injection of HBc DNA vaccine (pc -HBc) and intraperitoneal injection of HBc-transfected Renca and EL-4 effectively induced anti-HBc antibody response in mouse. Lipopolysaccharide (LPS) induced gene expressions of IP-10, GM-CSF, and IL-12 in dendritic cell (DC). However, LPS-stimulated DC or peritoneal macrophage, that were trasnsfected with HBc mRNA, on HBc mRNA vaccine itself induced lower anti-HBc antibody response in mouse compared to pc-HBc vaccine. These results suggest HBc DNA vaccine is more simple and effective for the induction of anti-HBc antibody response than HBc mRNA or mRNA-transfected cell vaccine.