미만성 대세포림프종의 Retinoblastoma 2/p130 유전자 분석

Abstract

A Retinoblastoma-related gene 2/p130 (pRb2/p130), which belongs to Rb family, was recently discovered. There were no studies about pRb2/p130 and mutation of tumor in contrast to this fact that pRb/p105 was related to various kinds of other cancer. There are many well known cancer genes, which are related to the diffuse large cell lymphoma (DLCL), but no researches have so far been made at the study of the mutation of pRb2/p130. Therefore, we have been made this study to show the features of mutation of pRb2/p130 in DLCL. For the research materials, we used the 10 frozen tissues, which were diagnosed as DLCL. Eight out of them were diffuse large B cell lymphoma (DLBL), and two were Burkitt’s lymphoma (BL). After we extracted the DNA from the frozen tissues, then we did PCR about the pRB/p130 exon 19, 20, 21, 22, after that, we directly analyzed the sequence of an amplified DNA. However, there were no mutation of pRb2/p130 in both DLBL 8 and BL 2. In this research, we can infer that there is no relationship between the cancer development of this tumor and the mutation of pRb2/p130 because there was no sign of mutation in the DLBL and sporadic BL and the exon 19, 20, 21, 22 of pRb2/p130. When we compare this with the results of a former research, which had suggested the existence of lots of mutations of pRb2/p130 in endemic (Burkitt’s lymphoma), we can find that the non-existence of mutation of pRb2/p130 at DLBL indicates that DLBL is different from the endemic and it is one of the characteristics which are different from the carcinogenesis and in the light of classification based on molecular biology.