흰쥐 성곽유두 맛봉오리의 퇴화와 재생과정에 따른 렉틴조직화학

Abstract

The present study was carried out to investigate the structure and glycoconjugate properties of degenerating and regenerating taste buds in the circumvallate papilla. The bilateral glossopharyngeal nerves of Sprague-Dawley male rats (300-350 g) were crushed by tweezers for 15 seconds. The animals were sacrificed 1, 4, 7, 10 days, 2, 3, 4 and 6 weeks later. The circumvallate papilla of the tongue was dissected, the tissues were then fixed with 4% paraformaldehyde solution, and tissue samples after hematoxylin – eosin staining were examined for morphological changes. They were also reacted with 8 biotinylated lectins: Con A, PSA, UEA I, ECL, DBA, HPA, SJA and sWGA. In the control, the taste buds in the circumvallate papilla were oval shape, and the two types of cells, round pale – staining nuclei and more slender darker – staining nuclei, were present in the taste buds. The taste buds and taste cells were extremely atrophied in the 4 days group, and a large number of the taste cells with round nuclei appeared in the 7 days group. The taste buds and taste cells regenerated in the 2 weeks group and they had the structure similar to the control in the 4 weeks group. In the control, strong positive reaction of taste pore and taste cells in the taste buds to UEA I, SBA, HPA and SJA may suggest that the taste cells secrete glycoconjugates which contained α-L-fucose and N-acetyl-D-galactosamine. the DBA, HPA and SJA positive reactive taste cells decreased in the 7 days group and increased in the 10 days and 4 weeks groups.