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흰쥐 성곽유두 맛봉오리의 퇴화와 재생과정에 따른 렉틴조직화학

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Author(s)
문용석김욱정효순최인장
Keimyung Author(s)
Choi, In Jang
Department
Dept. of Anatomy (해부학)
Journal Title
Keimyung Medical Journal
Issued Date
2003
Volume
22
Issue
2
Keyword
Circum vallate papillaDegenerationHistochemistryLectinRegenerationTaste bud
Abstract
The present study was carried out to investigate the structure and glycoconjugate properties of degenerating and regenerating taste buds in the circumvallate papilla. The bilateral glossopharyngeal nerves of Sprague-Dawley male rats (300-350 g) were crushed by tweezers for 15 seconds. The animals were sacrificed 1, 4, 7, 10 days, 2, 3, 4 and 6 weeks later. The circumvallate papilla of the tongue was dissected, the tissues were then fixed with 4% paraformaldehyde solution, and tissue samples after hematoxylin – eosin staining were examined for morphological changes. They were also reacted with 8 biotinylated lectins: Con A, PSA, UEA I, ECL, DBA, HPA, SJA and sWGA. In the control, the taste buds in the circumvallate papilla were oval shape, and the two types of cells, round pale – staining nuclei and more slender darker – staining nuclei, were present in the taste buds. The taste buds and taste cells were extremely atrophied in the 4 days group, and a large number of the taste cells with round nuclei appeared in the 7 days group. The taste buds and taste cells regenerated in the 2 weeks group and they had the structure similar to the control in the 4 weeks group. In the control, strong positive reaction of taste pore and taste cells in the taste buds to UEA I, SBA, HPA and SJA may suggest that the taste cells secrete glycoconjugates which contained α-L-fucose and N-acetyl-D-galactosamine. the DBA, HPA and SJA positive reactive taste cells decreased in the 7 days group and increased in the 10 days and 4 weeks groups.
Alternative Title
Lectin Histochemistry
of Degenerating and Regenerating Taste Buds in Rat
Circumvallate Papilla
Keimyung Author(s)(Kor)
최인장
Publisher
Keimyung University School of Medicine
Citation
문용석 et al. (2003). 흰쥐 성곽유두 맛봉오리의 퇴화와 재생과정에 따른 렉틴조직화학. Keimyung Medical Journal, 22(2), 186–194.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/15280
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 2003
1. School of Medicine (의과대학) > Dept. of Anatomy (해부학)
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