Methylthiazol Tetrazolium Assay를 이용한 소아백혈병환자 단핵세포의 항암제 감수성 검사

Abstract

Leukemia can be treated with various methods using diverse agents according to the prognostic factors. Chemosensitivity test was assessed with methylthiazol tetrazolium (MTT) assay. Mononuclear cells were harvested from the bone marrow of the leukemic patients and control group, then separated and incubated with 6 drugs for 96 hours. Then cells were incubated with MTT for 6 hours. Optical densities were calculated and LD50 (drug concentration lethal to 50% of the cells) were compared. Acute lymphoblastic leukemia (ALL) cells were more sensitive to cytarabine, etoposide and arsenic trioxide than acute myelocytic leukemia (AML) cells. In the age group of 2-10 year, ALL cells were more sensitive to etoposide and arsenic trioxide than AML cells and control group. In the age over 10 year group, ALL cells were not sensitive to any agents. AML cells showed sensitivite than cells from male patients. Cells were more sensitive to adriamycine, cytarabine, mistletoe extract and arsenic trioxide in the group whose initial WBC counts were less than 50,000/mm3 compared to the group whose initial WBC counts were over 50,000/mm3. Using MTT assay, author could compare and confirm the effect of conventional and alternative chemotherapeutic agents in pediatric leukemic cells.