일차배양 해마 신경세포에서 연에 의해 유도된 세포 독성

Abstract

This study carried out to demonstrate of relationships between lipid peroxidation and lead-induced neuronal cell toxicity by measure of the cellular toxicity index on various doses of lead, and to investigate the effect of lead on N-methyl-D-aspartate(NMDA), α-amino-3- hydroxy-5-methylisoxa zole-4-propionic acid(AMPA) and kainate-induced neurotoxicity. The cellular toxicity was assessed by morphological examination and by measuring the release of lacate dehydrogenase (LDH) and nitric oxide production. The cell viability of hippocampal neuronal cells showed to decrese following exposure to lead in a dose-dependent manner, resulting in 76.67% of cell viability at 1,000 μM, 12-hour lead treatment. The LDH leakage was significantly increased at 100 and 1,000 μM lead treatment for 12 hours. Nitric oxide production significantly increased at 1,000 μM lead treatment for 4 hours. Lead at 50 μM reduced significantly NMDA-induced cytotoxicity at 100 μM NMDA treatment, whereas it failed to reduce AMPA and kainate-induced cytotoxicity at 100 μM treatment respectively. On the basis of these results the cytotoxicity of hippocampus by lead is related with lipid peroxidation, lead reduced NMDA-induced cytotoxicity.