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Nerve Growth Factor로 분화된 PC12 세포에서 GABA 및 NMDA 수용체의 전기생리학적 특성

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Author(s)
송대규배재훈박원균
Alternative Author(s)
Song, Dae KyuBae, Jae HoonPark, Won Kyun
Issued Date
2002
Keyword
GABA receptorNerve growth factorNMDA receptorPC12 cells
Abstract
Nerve growth factor (NGF), which has been used for the differentiation
of PC12 cells in culture, not only promotes the survival and differentiation of
neurons but also affects the suructural and functional properties. The aim of this
study was to investigate the current properties of NMDA and GABA receptors by
using whole-cell patch clamp technique in NGF differentiated PC12 cells cultured
for 7~14 days. Membrane potential did not change from the resting potential of -
48 mV by the infusion of a NMDA receptor blocker, APV, (50 μM) in the perfusion
solution. NMDA components of the evoked currents at the membrane potential,
changing from -80 mV to -10 mV, showed a voltage dependency in the currentpotential
relationship. When action potential and glutamate receptors were
blocked, membrane potential was hyperpolarized by the infusion of GABA (20 μM)
in some PC12 cells, but not in other cells. In the hyperpolarized cells, GABA components
of the evoked currents at the membrane potential, changing from -80 mV
to -10 mV, showed a linear correlation between the currents and the membrnae
potential. In conclusion, the electrophysiological properties of NMDA and GABA
receptors in NGF differentiated PC12 cells may be similar to those in the biological
neurons. Therefore, it seems that PC12 cells appear to be suited for the studies
on function and signal transmission of these receptors.
Alternative Title
Electrophysiological
Properties of NMDA and GABA Receptors
in Nerve Growth Factor Differentiated PC12 Cells
Department
Dept. of Physiology (생리학)
Dept. of Medical Education (의학교육학)
Brain Research Institute (뇌연구소)
Publisher
Keimyung University School of Medicine
Citation
Keimyung Medical Journal, Vol.21(1) : 49, 2002
Type
Article
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/15964
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 2002
3. Research Institutues (연구소) > Brain Research Institute (뇌연구소)
1. School of Medicine (의과대학) > Dept. of Medical Education (의학교육학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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