Nerve Growth Factor로 분화된 PC12 세포에서 GABA 및 NMDA 수용체의 전기생리학적 특성

Abstract

Nerve growth factor (NGF), which has been used for the differentiation of PC12 cells in culture, not only promotes the survival and differentiation of neurons but also affects the suructural and functional properties. The aim of this study was to investigate the current properties of NMDA and GABA receptors by using whole-cell patch clamp technique in NGF differentiated PC12 cells cultured for 7~14 days. Membrane potential did not change from the resting potential of - 48 mV by the infusion of a NMDA receptor blocker, APV, (50 μM) in the perfusion solution. NMDA components of the evoked currents at the membrane potential, changing from -80 mV to -10 mV, showed a voltage dependency in the currentpotential relationship. When action potential and glutamate receptors were blocked, membrane potential was hyperpolarized by the infusion of GABA (20 μM) in some PC12 cells, but not in other cells. In the hyperpolarized cells, GABA components of the evoked currents at the membrane potential, changing from -80 mV to -10 mV, showed a linear correlation between the currents and the membrnae potential. In conclusion, the electrophysiological properties of NMDA and GABA receptors in NGF differentiated PC12 cells may be similar to those in the biological neurons. Therefore, it seems that PC12 cells appear to be suited for the studies on function and signal transmission of these receptors.