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일차 배양 해마신경세포에서 BDNF가 GABA성 전류에 미치는 영향

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Author(s)
송대규배재훈박원균
Alternative Author(s)
Song, Dae KyuBae, Jae HoonPark, Won Kyun
Issued Date
2002
Keyword
Brain-derived neurotrophic factorGABASynaptic current
Abstract
This study was performed to observe the effect of briefly (30-50 ms)
sprizing brain-derived neurotrophic factor (BDNF) on synaptic currents and to
evaluate the role of GABA receptor in this synaptic transmission. Thus rat
hippocampal neurons were prepared from 17∼18 day embryonic rats and cultured
for 7∼10 days to study the electrophysiological properties. BDNF inhibited the
frequency of spontaneous current and action potential within it's 2∼3 min, but
did not affect it's amplitudes. Membrane potential was slowly hyperpolarized. The
evoked current by a brief application of GABA (30∼50 ms) showed the maximum
peak at 100 μM and GABA. The feature and amplitude of evoked current were not
different before and after BDNF administration. This GABAergic evoked current
was completely inhibited by a GABAA receptor blocker, 100 μM bicuculline. When
the current-voltage curve of GABAergic current was plotted, short-term
administration of BDNF did not alter the property of GABA receptor. In
conclusion, it is suggested that BDNF may hyperpolarize the membrane potential
by activation of GABAergic synaptic terminal of early developing hippocampal
neurons and inhibit the frequency of synaptic currents and action potential.
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