일차 배양 해마신경세포에서 BDNF가 GABA성 전류에 미치는 영향

Abstract

This study was performed to observe the effect of briefly (30-50 ms) sprizing brain-derived neurotrophic factor (BDNF) on synaptic currents and to evaluate the role of GABA receptor in this synaptic transmission. Thus rat hippocampal neurons were prepared from 17∼18 day embryonic rats and cultured for 7∼10 days to study the electrophysiological properties. BDNF inhibited the frequency of spontaneous current and action potential within it's 2∼3 min, but did not affect it's amplitudes. Membrane potential was slowly hyperpolarized. The evoked current by a brief application of GABA (30∼50 ms) showed the maximum peak at 100 μM and GABA. The feature and amplitude of evoked current were not different before and after BDNF administration. This GABAergic evoked current was completely inhibited by a GABAA receptor blocker, 100 μM bicuculline. When the current-voltage curve of GABAergic current was plotted, short-term administration of BDNF did not alter the property of GABA receptor. In conclusion, it is suggested that BDNF may hyperpolarize the membrane potential by activation of GABAergic synaptic terminal of early developing hippocampal neurons and inhibit the frequency of synaptic currents and action potential.