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일차 배양 해마신경세포에서 BDNF가 GABA성 전류에 미치는 영향

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Affiliated Author(s)
송대규배재훈박원균
Alternative Author(s)
Song, Dae KyuBae, Jae HoonPark, Won Kyun
Journal Title
Keimyung Medical Journal
Issued Date
2002
Keyword
Brain-derived neurotrophic factorGABASynaptic current
Abstract
This study was performed to observe the effect of briefly (30-50 ms)
sprizing brain-derived neurotrophic factor (BDNF) on synaptic currents and to
evaluate the role of GABA receptor in this synaptic transmission. Thus rat
hippocampal neurons were prepared from 17∼18 day embryonic rats and cultured
for 7∼10 days to study the electrophysiological properties. BDNF inhibited the
frequency of spontaneous current and action potential within it's 2∼3 min, but
did not affect it's amplitudes. Membrane potential was slowly hyperpolarized. The
evoked current by a brief application of GABA (30∼50 ms) showed the maximum
peak at 100 μM and GABA. The feature and amplitude of evoked current were not
different before and after BDNF administration. This GABAergic evoked current
was completely inhibited by a GABAA receptor blocker, 100 μM bicuculline. When
the current-voltage curve of GABAergic current was plotted, short-term
administration of BDNF did not alter the property of GABA receptor. In
conclusion, it is suggested that BDNF may hyperpolarize the membrane potential
by activation of GABAergic synaptic terminal of early developing hippocampal
neurons and inhibit the frequency of synaptic currents and action potential.
Alternative Title
Effect of BDNF on
GABAergic Currents in
Primary Cultured Rat Hippocampal Neurons
Department
Dept. of Physiology (생리학)
Dept. of Medical Education (의학교육학)
Brain Research Institute (뇌연구소)
Publisher
Keimyung University School of Medicine
Citation
황진수 et al. (2002). 일차 배양 해마신경세포에서 BDNF가 GABA성 전류에 미치는 영향. Keimyung Medical Journal, 21(2), 155–168.
Type
Article
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/15966
Appears in Collections:
2. Keimyung Medical Journal (계명의대 학술지) > 2002
3. Research Institutues (연구소) > Brain Research Institute (뇌연구소)
1. School of Medicine (의과대학) > Dept. of Medical Education (의학교육학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
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