Bovine colostrum inhibits nuclear factor κB–mediated proinflammatory cytokine expression in intestinal epithelial cells
- Affiliated Author(s)
- 김은수
- Alternative Author(s)
- Kim, Eun Soo
- Journal Title
- Nutrition Research
- ISSN
- 0271-5317
- Issued Date
- 2009
- Keyword
- Bovine; Colostrum; NF-κB; Intestinal; Inflammation; HT-29 cells
- Abstract
- Colostrum, a nutrient-rich fluid produced by female mammals immediately after giving birth, is loaded with several immune, growth, and tissue repair factors. However, it remains unknown whether bovine colostrum has anti-inflammatory effects on intestinal epithelial cells (IEC). In this study, we aimed to investigate the anti-inflammatory effects of colostrum on IEC and to elucidate its molecular mechanisms. Human colon cancer HT-29 cells were stimulated with interleukin (IL)–1β with or without bovine colostrum. The effects of colostrum on nuclear factor κB (NF-κB) signaling in HT-29 cells were examined using real-time reverse transcriptase–polymerase chain reaction detect IL-8 and intracellar adhesion molecule-1 mRNA expression using a NF-κB-dependent reporter gene assay and an electrophoretic mobility shift assay. Furthermore, we assessed the expression levels of inhibitor protein of NF-κB–α, cyclooxygenase-2, and p65 proteins by Western blotting. Bovine colostrum significantly inhibited IL-1β-induced IL-8 and intracellar adhesion molecule-1 mRNA expression. Moreover, it suppressed IL-1β-induced NF-κB activation, including NF-κB dependent reporter gene expression in a dose-dependent manner. Finally, Western blotting revealed that colostrum decreased the cyclooxygenase-2 protein expression level, inhibited inhibitor protein of NF-κB–α degradation, and blocked translocation of p65 into the nucleus. These data demonstrated that bovine colostrum might protect against IEC inflammation by inhibiting the NF-κB pathway, suggesting colostrum has a therapeutic potential for intestinal inflammation.
Abbreviations
NF-κB, nuclear factor κB;
IEC, intestinal epithelial cell;
IL, interleukin;
FBS, fetal bovine serum;
EMSA, electrophoretic mobility shift assay;
IκB, inhibitor of NF-κB;
COX-2, cyclooxygenase-2;
ICAM-1, intracellar adhesion molecule 1;
TNF, tumor necrosis factor
Keywords
Bovine;
Colostrum;
NF-κB;
Intestinal;
Inflammation;
HT-29 cells
- 공개 및 라이선스
-
- 파일 목록
-
Items in Repository are protected by copyright, with all rights reserved, unless otherwise indicated.