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Bovine colostrum inhibits nuclear factor κB–mediated proinflammatory cytokine expression in intestinal epithelial cells

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Author(s)
Min Ji AnJae Hee CheonSeung Won KimJae Jun ParkChang Mo MoonSong Yi HanEun Soo KimTae Il KimWon Ho Kim
Keimyung Author(s)
Kim, Eun Soo
Department
Dept. of Internal Medicine (내과학)
Journal Title
Nutrition Research
Issued Date
2009
Volume
29
Issue
4
Keyword
BovineColostrumNF-κBIntestinalInflammationHT-29 cells
Abstract
Colostrum, a nutrient-rich fluid produced by female mammals immediately after giving birth, is loaded with several immune, growth, and tissue repair factors. However, it remains unknown whether bovine colostrum has anti-inflammatory effects on intestinal epithelial cells (IEC). In this study, we aimed to investigate the anti-inflammatory effects of colostrum on IEC and to elucidate its molecular mechanisms. Human colon cancer HT-29 cells were stimulated with interleukin (IL)–1β with or without bovine colostrum. The effects of colostrum on nuclear factor κB (NF-κB) signaling in HT-29 cells were examined using real-time reverse transcriptase–polymerase chain reaction detect IL-8 and intracellar adhesion molecule-1 mRNA expression using a NF-κB-dependent reporter gene assay and an electrophoretic mobility shift assay. Furthermore, we assessed the expression levels of inhibitor protein of NF-κB–α, cyclooxygenase-2, and p65 proteins by Western blotting. Bovine colostrum significantly inhibited IL-1β-induced IL-8 and intracellar adhesion molecule-1 mRNA expression. Moreover, it suppressed IL-1β-induced NF-κB activation, including NF-κB dependent reporter gene expression in a dose-dependent manner. Finally, Western blotting revealed that colostrum decreased the cyclooxygenase-2 protein expression level, inhibited inhibitor protein of NF-κB–α degradation, and blocked translocation of p65 into the nucleus. These data demonstrated that bovine colostrum might protect against IEC inflammation by inhibiting the NF-κB pathway, suggesting colostrum has a therapeutic potential for intestinal inflammation.

Abbreviations
NF-κB, nuclear factor κB;
IEC, intestinal epithelial cell;
IL, interleukin;
FBS, fetal bovine serum;
EMSA, electrophoretic mobility shift assay;
IκB, inhibitor of NF-κB;
COX-2, cyclooxygenase-2;
ICAM-1, intracellar adhesion molecule 1;
TNF, tumor necrosis factor

Keywords
Bovine;
Colostrum;
NF-κB;
Intestinal;
Inflammation;
HT-29 cells
Keimyung Author(s)(Kor)
김은수
Publisher
School of Medicine
Citation
Min Ji An et al. (2009). Bovine colostrum inhibits nuclear factor κB–mediated proinflammatory cytokine expression in intestinal epithelial cells. Nutrition Research, 29(4), 275–280. doi: 10.1016/j.nutres.2009.03.011
Type
Article
ISSN
0271-5317
Source
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S0271531709000554?via%3Dihub
DOI
10.1016/j.nutres.2009.03.011
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/33581
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
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