Aspirin induces apoptosis in YD-8 human oral squamous carcinoma cells through activation of caspases, down-regulation of Mcl-1, and inactivation of ERK-1/2 and AKT

Authors
In-Sook ParkJeong-Rang JoHua HongKi-Young NamJong-Bae KimSang-Hee HwangMi-Sun ChoiNam-Hee RyuHyun-Jung JangSang-Han LeeChin-Soo KimTae-Geon KwonGy-Young ParkJong-Wook ParkByeong-Churl Jang
Department
Dept. of Dentistry (치과학); Dept. of Molecular Medicine (분자의학); Dept. of Immunology (면역학); Dept. of Pathology (병리학); Dept. of Laboratory Medicine (진단검사의학)
Issue Date
2010
Citation
Toxicol In Vitro, Vol.24(3) : 713-720, 2010
ISSN
0887-2333
Abstract
NSAIDs and COX-2 inhibitors show anti-cancer activities in many cancer cells. In this study, we investi- gated the effects of NSAIDs (aspirin or indomethacin) and COX-2 inhibitor (NS-398) on growth of YD-8 human oral squamous carcinoma cells. Interestingly, among drugs tested, aspirin showed strongest inhibitory effects on viability and survival of YD-8 cells. Profoundly, aspirin treatment resulted in severe cell shrinkage and nuclear DNA fragmentation in YD-8 cells, suggesting the aspirin-induced apoptosis in YD-8 cells. Data of Western blot further demonstrated that aspirin treatment caused activation of casp- ases, down-regulation of Mcl-1 protein, dephosphorylation of ERK-1/2 and AKT, and also IjB-a proteol- ysis-dependent NF-jB activation in YD-8 cells. Aspirin, however, had no effect on expressions of Bcl-2, XIAP, and HIAP-1 in YD-8 cells. Importantly, pretreatment with z-VAD-fmk, a pan-caspase inhibitor blocked the aspirin-induced apoptosis and Mcl-1 down-regulation in YD-8 cells. These findings collec- tively suggest that aspirin induces apoptosis in YD-8 cells and the induction may be correlated to activa- tion of caspases, caspase-dependent Mcl-1 proteolysis, inactivation of ERK-1/2 and AKT, and activation of NF-jB. It is suggested that aspirin may be applied a potential anti-cancer drug against human oral squa- mous carcinoma. 2010 Elsevier Ltd. All rights reserved. Keywords: Aspirin Apoptosis YD-8 OSC cells Caspases Mcl-1 ERK-1/2 AKT NF-jB
Keywords
AspirinApoptosisYD-8 OSC cellsCaspasesMcl-1ERK-1/2AKTNF-κB
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/34666
Appears in Collections:
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Dentistry (치과학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
1. Journal Papers (연구논문) > 1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
Keimyung Author(s)
남기영; 김종배; 황상희; 박종욱; 장병철; 최미선; 류남희
Full Text
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S0887233310000123?via%3Dihub
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