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Aspirin induces apoptosis in YD-8 human oral squamous carcinoma cells through activation of caspases, down-regulation of Mcl-1, and inactivation of ERK-1/2 and AKT

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Author(s)
In-Sook ParkJeong-Rang JoHua HongKi-Young NamJong-Bae KimSang-Hee HwangMi-Sun ChoiNam-Hee RyuHyun-Jung JangSang-Han LeeChin-Soo KimTae-Geon KwonGy-Young ParkJong-Wook ParkByeong-Churl Jang
Keimyung Author(s)
Nam, Ki YoungKim, Jong BaeHwang, Sang HeePark, Jong WookJang, Byeong ChurlChoe, Mi SunRyoo, Nam Hee
Department
Dept. of Dentistry (치과학)
Dept. of Molecular Medicine (분자의학)
Dept. of Immunology (면역학)
Dept. of Pathology (병리학)
Dept. of Laboratory Medicine (진단검사의학)
Journal Title
Toxicol In Vitro
Issued Date
2010
Volume
24
Issue
3
Keyword
AspirinApoptosisYD-8 OSC cellsCaspasesMcl-1ERK-1/2AKTNF-κB
Abstract
NSAIDs and COX-2 inhibitors show anti-cancer activities in many cancer cells. In this study, we investi-
gated the effects of NSAIDs (aspirin or indomethacin) and COX-2 inhibitor (NS-398) on growth of YD-8
human oral squamous carcinoma cells. Interestingly, among drugs tested, aspirin showed strongest
inhibitory effects on viability and survival of YD-8 cells. Profoundly, aspirin treatment resulted in severe
cell shrinkage and nuclear DNA fragmentation in YD-8 cells, suggesting the aspirin-induced apoptosis in
YD-8 cells. Data of Western blot further demonstrated that aspirin treatment caused activation of casp-
ases, down-regulation of Mcl-1 protein, dephosphorylation of ERK-1/2 and AKT, and also IjB-a proteol-
ysis-dependent NF-jB activation in YD-8 cells. Aspirin, however, had no effect on expressions of Bcl-2,
XIAP, and HIAP-1 in YD-8 cells. Importantly, pretreatment with z-VAD-fmk, a pan-caspase inhibitor
blocked the aspirin-induced apoptosis and Mcl-1 down-regulation in YD-8 cells. These findings collec-
tively suggest that aspirin induces apoptosis in YD-8 cells and the induction may be correlated to activa-
tion of caspases, caspase-dependent Mcl-1 proteolysis, inactivation of ERK-1/2 and AKT, and activation of
NF-jB. It is suggested that aspirin may be applied a potential anti-cancer drug against human oral squa-
mous carcinoma.
2010 Elsevier Ltd. All rights reserved. Keywords:
Aspirin
Apoptosis
YD-8 OSC cells
Caspases
Mcl-1
ERK-1/2
AKT
NF-jB
Keimyung Author(s)(Kor)
남기영
김종배
황상희
박종욱
장병철
최미선
류남희
Publisher
School of Medicine
Citation
In-Sook Park et al. (2010). Aspirin induces apoptosis in YD-8 human oral squamous carcinoma cells through activation of caspases, down-regulation of Mcl-1, and inactivation of ERK-1/2 and AKT. Toxicol In Vitro, 24(3), 713–720. doi: 10.1016/j.tiv.2010.01.010
Type
Article
ISSN
0887-2333
Source
http://lps3.www.sciencedirect.com.proxy.dsmc.or.kr/science/article/pii/S0887233310000123?via%3Dihub
DOI
10.1016/j.tiv.2010.01.010
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34666
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Dentistry (치과학)
1. School of Medicine (의과대학) > Dept. of Immunology (면역학)
1. School of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학)
1. School of Medicine (의과대학) > Dept. of Molecular Medicine (분자의학)
1. School of Medicine (의과대학) > Dept. of Pathology (병리학)
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