Effects of Cyclosporine and Tacrolimus on the Oxidative Stress in Cultured Mesangial Cells
- 서성일; 문교철; 곽춘식; 배재훈; 한승엽; 박성배; 김현철
- Alternative Author(s)
- Suh, Seong Il; Mun, Kyo Cheol; Kwak, Chun Sik; Bae, Jae Hoon; Han, Seung Yeup; Park, Sung Bae; Kim, Hyun Chul
- Publication Year
- Objectives. Cyclosporine (CsA) and tacrolimus (Tac) are two primary immunosuppres-
sive agents used for the prevention of graft rejection. However, their use is associated with
signiﬁcant side effects, most notably nephrotoxicity. The mechanisms of this toxicity are not
fully understood, but they seem to be associated with increases in the production of oxygen
free radicals (OFRs). This present work examined the effect of CsA and Tac on the
production of OFRs in cultured rat renal mesangial cells (RMCs).
Methods. Varying concentrations of CsA and Tac (0 to 40 mol/L) were added to RMCs
and incubated for 60 minutes at 37°C. The production of OFRs was evaluated by measuring
the ﬂuorescent product from the oxidation of an oxidant-sensitive 2=,7=-dichloroﬂuorescin.
Results. At 60 minutes, the relative ﬂuorescence units (RFU) for OFRs production in
RMCs exposure to CsA were increased by 2.5%, 11.5%, 22.5%, 57.2%, and 174% at 2.5,
5, 10, 20, and 40 mol/L, respectively. Tac increased the RFU by 15.9%, 13.6%, 14.8%,
13.2%, 21.4%, 13.2%, and 28.1% at 0.1, 1, 2.5, 5, 10, 20, and 40 mol/L, respectively. In
RMCs, the RFU produced by CsA was higher than that by Tac.
Conclusions. The results of this experiment suggest that CsA and Tac induced renal
injury by OFRs.
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