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Effects of Cyclosporine and Tacrolimus on the Oxidative Stress in Cultured Mesangial Cells

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Author(s)
S.Y. HanK.C. MunH.J. ChoiC.S. KwakJ.H. BaeS.I. SuhS.B. ParkH.C. KimE.J. Chang
Keimyung Author(s)
Suh, Seong IlMun, Kyo CheolKwak, Chun SikBae, Jae HoonHan, Seung YeupPark, Sung BaeKim, Hyun Chul
Department
Dept. of Microbiology (미생물학)
Dept. of Biochemistry (생화학)
Dept. of Physiology (생리학)
Dept. of Internal Medicine (내과학)
Kidney Institute (신장연구소)
Journal Title
Transplantation Proceedings
Issued Date
2006
Volume
38
Issue
7
Abstract
Objectives. Cyclosporine (CsA) and tacrolimus (Tac) are two primary immunosuppres-
sive agents used for the prevention of graft rejection. However, their use is associated with
significant side effects, most notably nephrotoxicity. The mechanisms of this toxicity are not
fully understood, but they seem to be associated with increases in the production of oxygen
free radicals (OFRs). This present work examined the effect of CsA and Tac on the
production of OFRs in cultured rat renal mesangial cells (RMCs).
Methods. Varying concentrations of CsA and Tac (0 to 40 mol/L) were added to RMCs
and incubated for 60 minutes at 37°C. The production of OFRs was evaluated by measuring
the fluorescent product from the oxidation of an oxidant-sensitive 2=,7=-dichlorofluorescin.
Results. At 60 minutes, the relative fluorescence units (RFU) for OFRs production in
RMCs exposure to CsA were increased by 2.5%, 11.5%, 22.5%, 57.2%, and 174% at 2.5,
5, 10, 20, and 40 mol/L, respectively. Tac increased the RFU by 15.9%, 13.6%, 14.8%,
13.2%, 21.4%, 13.2%, and 28.1% at 0.1, 1, 2.5, 5, 10, 20, and 40 mol/L, respectively. In
RMCs, the RFU produced by CsA was higher than that by Tac.
Conclusions. The results of this experiment suggest that CsA and Tac induced renal
injury by OFRs.
Keimyung Author(s)(Kor)
서성일
문교철
곽춘식
배재훈
한승엽
박성배
김현철
Publisher
School of Medicine
Citation
S.Y. Han et al. (2006). Effects of Cyclosporine and Tacrolimus on the Oxidative Stress in Cultured Mesangial Cells. Transplantation Proceedings, 38(7), 2240–2241. doi: 10.1016/j.transproceed.2006.06.078
Type
Article
ISSN
0041-1345
Source
https://www.sciencedirect.com/science/article/pii/S0041134506007007?via%3Dihub
DOI
10.1016/j.transproceed.2006.06.078
URI
https://kumel.medlib.dsmc.or.kr/handle/2015.oak/34677
Appears in Collections:
1. School of Medicine (의과대학) > Dept. of Biochemistry (생화학)
1. School of Medicine (의과대학) > Dept. of Internal Medicine (내과학)
1. School of Medicine (의과대학) > Dept. of Microbiology (미생물학)
1. School of Medicine (의과대학) > Dept. of Physiology (생리학)
3. Research Institutues (연구소) > Kidney Institute (신장연구소)
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