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Cyclosporine-Induced Apoptosis in Osteosarcoma Cells

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Author(s)
한승엽황은아박성배김현철
Alternative Author(s)
Han, Seung YeupHwang, Eun AhPark, Sung BaeKim, Hyun Chul
Publication Year
2006
Abstract
Introduction. Posttransplant bone disease is one of the complications of cyclosporine
(CsA), which is widely used as an immunosuppressive agent in the field of kidney
transplantation. Cyclosporine treatment causes osteopenia as a result of altered bone
turnover, but the pathogenic mechanisms of this process remain unclear. This study
examined the ability of CsA to induce apoptosis in a rat osteoblast cell line.
Results. We induced apoptosis in rat osteoblastic ROS 17/2.8 cells by exposure to CsA.
MTT assay showed that CsA exhibited significant cytotoxic effects on ROS 17/2.8 cells in a
dose-dependent manner.Western blot analysis showed enhanced processing of caspase-8, Bax,
and p53 after CsA treatment. Expression of cleaved poly (ADP-ribose) polymerase (PARP)
was elevated by CsA treatment. Pro-caspase-3 and Bcl-2 proteins were decreased by CsA.
Conclusions. These results suggested that CsA induced apoptosis of osteoblasts.
Department
Dept. of Internal Medicine (내과학)
Kidney Institute (신장연구소)
Publisher
School of Medicine
Citation
Transplantation proceeding, Vol.38(7) : 2237-2239, 2006
Type
Article
ISSN
0041-1345
DOI
10.1016/j.transproceed.2006.06.025
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/34716
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