Cyclosporine-Induced Apoptosis in Osteosarcoma Cells
- 한승엽; 황은아; 박성배; 김현철
- Alternative Author(s)
- Han, Seung Yeup; Hwang, Eun Ah; Park, Sung Bae; Kim, Hyun Chul
- Publication Year
- Introduction. Posttransplant bone disease is one of the complications of cyclosporine
(CsA), which is widely used as an immunosuppressive agent in the ﬁeld of kidney
transplantation. Cyclosporine treatment causes osteopenia as a result of altered bone
turnover, but the pathogenic mechanisms of this process remain unclear. This study
examined the ability of CsA to induce apoptosis in a rat osteoblast cell line.
Results. We induced apoptosis in rat osteoblastic ROS 17/2.8 cells by exposure to CsA.
MTT assay showed that CsA exhibited signiﬁcant cytotoxic effects on ROS 17/2.8 cells in a
dose-dependent manner.Western blot analysis showed enhanced processing of caspase-8, Bax,
and p53 after CsA treatment. Expression of cleaved poly (ADP-ribose) polymerase (PARP)
was elevated by CsA treatment. Pro-caspase-3 and Bcl-2 proteins were decreased by CsA.
Conclusions. These results suggested that CsA induced apoptosis of osteoblasts.
- Authorize & License
- Files in This Item:
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.