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Effects of Cyclosporine on the Production of the Reactive Oxygen Species in the Glial Cells

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Author(s)
문교철하은영김여희진규복한승엽박성배김현철
Alternative Author(s)
Mun, Kyo CheolHa, Eun YoungKim, You HeeJin, Kyu BokHan, Seung YeupPark, Sung BaeKim, Hyun Chul
Publication Year
2008
Abstract
Objectives

After organ transplantation, some patients suffer mild neurological symptoms such as tremor to severe complications including seizures and encephalopathy. Among the immunosuppressants, cyclosporine (CsA) can induce neurological side effects. However, the mechanisms of encephalopathy by CsA are not fully understood. We measured the production of reactive oxygen species (ROS) in the glioma cells after CsA treatment.

Methods

CsA (2.5 mmol/L) added to glioma cells was incubated for 60 minutes at 37°C. ROS production was evaluated by measuring the fluorescent product from the oxidation of an oxidant-sensitive 2′,7′-dichlorofluorescin using VICTOR3 multilabel counter.

Results

CsA resulted in ROS production by glioma cells. The ROS production increased with the time of exposure to CsA.

Conclusions

These findings indicated that CsA may contribute to neurological side effects via ROS production.
Department
Dept. of Biochemistry (생화학)
Dept. of Internal Medicine (내과학)
Kidney Institute (신장연구소)
Publisher
School of Medicine
Citation
Transplantation Proceedings, Vol.40(8) : 2742~2743-2742~2743, 2008
Type
Article
ISSN
0041-1345
DOI
10.1016/j.transproceed.2008.08.007
URI
http://kumel.medlib.dsmc.or.kr/handle/2015.oak/34743
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