Effects of Cyclosporine on the Production of the Reactive Oxygen Species in the Glial Cells

Abstract

Objectives

After organ transplantation, some patients suffer mild neurological symptoms such as tremor to severe complications including seizures and encephalopathy. Among the immunosuppressants, cyclosporine (CsA) can induce neurological side effects. However, the mechanisms of encephalopathy by CsA are not fully understood. We measured the production of reactive oxygen species (ROS) in the glioma cells after CsA treatment.

Methods

CsA (2.5 mmol/L) added to glioma cells was incubated for 60 minutes at 37°C. ROS production was evaluated by measuring the fluorescent product from the oxidation of an oxidant-sensitive 2′,7′-dichlorofluorescin using VICTOR3 multilabel counter.

Results

CsA resulted in ROS production by glioma cells. The ROS production increased with the time of exposure to CsA.

Conclusions

These findings indicated that CsA may contribute to neurological side effects via ROS production.